Article
Oligonucleotide-Mediated Genome Editing Provides Precision and Function to Engineered Nucleases and Antibiotics in Plants.
Plant physiology - 1 Apr 2016
Sauer Noel J, Narváez-Vásquez Javier, Mozoruk Jerry, Miller Ryan B, Warburg Zachary J, Woodward Melody J, Mihiret Yohannes A, Lincoln Tracey A, Segami Rosa E, Sanders Steven L, Walker Keith A, Beetham Peter R, Schöpke Christian R, Gocal Greg F W
Abstract excerpt
Here, we report a form of oligonucleotide-directed mutagenesis for precision genome editing in plants that uses single-stranded oligonucleotides (ssODNs) to precisely and efficiently generate genome edits at DNA strand lesions made by DNA double strand break reagents. Employing a transgene model in Arabidopsis (Arabidopsis thaliana), we obtained a high frequency of precise targeted genome edits when ssODNs were...
Topics
- 3-Phosphoshikimate 1-Carboxyvinyltransferase
- Adaptation, Physiological
- Alleles
- Anti-Bacterial Agents
- Arabidopsis
- Base Sequence
- CRISPR-Cas Systems
- Endonucleases
- Flax