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Article

Targeted Mutagenesis in Plant Cells through Transformation of Sequence-Specific Nuclease mRNA.

PloS one - 1 Jan 2016

Stoddard Thomas J, Clasen Benjamin M, Baltes Nicholas J, Demorest Zachary L, Voytas Daniel F, Zhang Feng, Luo Song

Abstract excerpt

Plant genome engineering using sequence-specific nucleases (SSNs) promises to advance basic and applied plant research by enabling precise modification of endogenous genes. Whereas DNA is an effective means for delivering SSNs, DNA can integrate randomly into the plant genome, leading to unintentional gene inactivation. Further, prolonged expression of SSNs from DNA constructs can lead to the accumulation of...

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Identifiers

PMID: 27176769
DOI: 10.1371/journal.pone.0154634
PMCID: PMC4866682

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Source receipt: pubmed, retrieved 7 Sept 2026.

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