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Article

The reliability of in vivo structure-function analysis of tRNA aminoacylation.

Journal of molecular biology - 9 Jul 1999

McClain W H, Jou Y Y, Bhattacharya S, Gabriel K, Schneider J

Abstract excerpt

The G.U wobble base-pair in the acceptor helix of Escherichia coli tRNAAlais critical for aminoacylation by the alanine synthetase. Previous work by several groups probed the mechanism of enzyme recognition of G.U by a structure-function analysis of mutant tRNAs using either a cell assay (amber suppressor tRNA) or a test tube assay (phage T7 tRNA substrate and purified enzyme). However, the aminoacylation...

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Source

Identifiers

PMID: 10390340
DOI: 10.1006/jmbi.1999.2884

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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