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Article

Modulation of tRNAAla identity by inorganic pyrophosphatase.

Proceedings of the National Academy of Sciences of the United States of America - 30 Apr 2002

Wolfson Alexey D, Uhlenbeck Olke C

Abstract excerpt

A highly sensitive assay of tRNA aminoacylation was developed that directly measures the fraction of aminoacylated tRNA by following amino acid attachment to the 3'-(32)P-labeled tRNA. When applied to Escherichia coli alanyl-tRNA synthetase, the assay allowed accurate measurement of aminoacylation of the most deleterious mutants of tRNA(Ala). The effect of tRNA(Ala) identity mutations on both aminoacylation...

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Identifiers

PMID: 11983895
DOI: 10.1073/pnas.092152799
PMCID: PMC122885

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Source receipt: pubmed, retrieved 7 Sept 2026.

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