Direct analysis of aminoacylation levels of tRNAs in vivo. Application to studying recognition of Escherichia coli initiator tRNA mutants by glutaminyl-tRNA synthetase.

Abstract excerpt

We describe the use of a gel electrophoretic method for measuring the levels of aminoacylation in vivo of mutant Escherichia coli initiator tRNAs, which are substrates for E. coli glutaminyl-tRNA synthetase (GlnRS) due to an anticodon sequence change. Using this method, we have compared the effec...

Topics

• Acylation
• Amines
• Base Sequence
• Blotting, Northern
• Codon
• DNA, Bacterial
• Electrophoresis, Polyacrylamide Gel
• Escherichia coli
• Genes, Bacterial
• Glutamate-tRNA Ligase
• Kinetics
• Molecular Sequence Data
• Mutation
• Nucleic Acid Conformation
• Peptide Chain Initiation, Translational
• RNA, Transfer