Sequence-dependent mutations in a shuttle vector plasmid replicated in a mismatch repair deficient human cell line.

doi:10.1093/carcin/20.7.1293

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Article

Sequence-dependent mutations in a shuttle vector plasmid replicated in a mismatch repair deficient human cell line.

Carcinogenesis - 1 Jul 1999

Tobi S E, Levy D D, Seidman1 M M, Kraemer1 K H

Abstract excerpt

We utilized a shuttle vector plasmid (pLSC) to assess the role of DNA sequence and mismatch repair on mutagenesis in human cells. pLSC contains an interrupted 29 bp mononucleotide poly(G) run within a bacterial suppressor tRNA gene, which acts as a highly sensitive mutagenic target for detection of base substitution and frameshift mutations. The frequency of spontaneous mutations in pLSC was found to be similar...

Topics

Base Pair Mismatch
Base Sequence
Cell Line
DNA Mutational Analysis
DNA Repair
DNA Replication
Genes, Suppressor
Genetic Vectors
Humans
Molecular Sequence Data
Mutagenesis
Mutation
Plasmids
RNA, Transfer
Sequence Deletion
Ultraviolet Rays

Source

Open in PubMed

Identifiers

PMID: 10383903
DOI: 10.1093/carcin/20.7.1293

Provenance

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