Article
N-methyl-N-nitrosourea-induced mutations in a shuttle plasmid replicated in human cells.
Molecular carcinogenesis - 1 Jan 1990
Sikpi M O, Waters L C, Kraemer K H, Preston R J, Mitra S
Abstract excerpt
The supF gene of the recombinant shuttle plasmid pZ190 (modified pZ189) was used as a target to study the nature of mutations induced by N-methyl-N-nitrosourea (MNU) in human cells. Treatment of the intact plasmid with MNU followed by its replication in human lymphoblastoid cells led to extensive inactivation and no detectable mutations of the plasmid. However, exposure of the supF DNA fragment alone, followed by...
Topics
- Bacterial Proteins
- Base Sequence
- Cell Line
- DNA Replication
- Female
- Genes, Bacterial
- Humans
- Methylnitrosourea
- Molecular Sequence Data
- Mutation
- Plasmids
- Transfection