Mutations induced by ionizing radiation in a plasmid replicated in human cells. I. Similar, nonrandom distribution of mutations in unirradiated and X-irradiated DNA.

Abstract excerpt

The Escherichia coli supF gene encoding the suppressor tyrosine tRNA in a human shuttle plasmid, pZ189, was used as a target for molecular analysis of X-ray-induced mutations in human lymphoblastoid cells. Following replication of the in vitro-irradiated plasmid in human cells, the mutant supF-containing molecules were cloned by phenotypic screening in E. coli and the nature of the mutations was determined by...

Topics

• Base Composition
• Base Sequence
• DNA
• DNA Damage
• Dose-Response Relationship, Radiation
• Humans
• Molecular Sequence Data
• Mutation
• Plasmids
• RNA, Transfer, Tyr
• X-Rays