Article
Use of DNA toolbox for the characterization of mutation scanning methods. II: evaluation of single-strand conformation polymorphism analysis.
Electrophoresis - 1 Jun 1999
Highsmith W E, Nataraj A J, Jin Q, O'Connor J M, El-Nabi S H, Kusukawa N, Garner M M
Abstract excerpt
Single-strand conformation polymorphism (SSCP) is one of the most commonly used methods for searching for unknown base changes (mutations). In order to characterize systematically the effects of important physical parameters on the sensitivity and specificity of SSCP, we used the DNA toolbox constructed as described in the companion paper [2]. Using this set of DNA molecules as polymerase chain reaction (PCR)...
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