Article
Use of a DNA toolbox for the characterization of mutation scanning methods. I: construction of the toolbox and evaluation of heteroduplex analysis.
Electrophoresis - 1 Jun 1999
Highsmith W E, Jin Q, Nataraj A J, O'Connor J M, Burland V D, Baubonis W R, Curtis F P, Kusukawa N, Garner M M
Abstract excerpt
A systematic characterization of the effects of important physical parameters on the sensitivity and specificity of methods in searching for unknown base changes (mutations or single nucleotide polymorphisms) over a relatively long DNA segment has not been previously reported. To this end, we have constructed a set of molecules of varying G+C content (40, 50, and 60% GC) having all possible base changes at a...
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