Article
A streamlined mutation detection system: multicolor post-PCR fluorescence labeling and single-strand conformational polymorphism analysis by capillary electrophoresis.
Genome research - 1 Nov 1997
Inazuka M, Wenz H M, Sakabe M, Tahira T, Hayashi K
Abstract excerpt
Effective use of knowledge of human genome sequences in studies of hereditary diseases or cancer heavily depends on efficient methods for detection of mutations in individual samples. We describe here a simple and efficient mutation scanning system in which PCR products are post-labeled with two...
Topics
- Automation
- Calibration
- Chromosome Mapping
- DNA Gyrase
- DNA Mutational Analysis
- DNA Topoisomerases, Type II
- Electrophoresis, Capillary
- Exons
- Genome, Human
- Humans
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Sensitivity and Specificity