Article
Ca²+-binding properties and regulatory roles of lobster troponin C sites II and IV.
FEBS letters - 19 Aug 2013
Tanaka Hiroyuki, Takahashi Hiroki, Ojima Takao
Abstract excerpt
Invertebrate troponin C typically contains Ca²⁺-specific binding sites, sites II and IV, in the N- and C-terminal domains, respectively. To investigate the roles of these sites for Ca²⁺-dependent regulation of muscle contraction, we generated lobster troponin C mutants, and analyzed their Ca²⁺-binding properties and regulatory effects on actomyosin-tropomyosin Mg-ATPase activity. The results suggest that Ca²⁺...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Calcium
- Magnesium
- Molecular Sequence Data
- Muscle Contraction
- Mutation
- Nephropidae
- Protein Binding
- Protein Isoforms
- Recombinant Proteins