Article
Functional importance of Ca2+-deficient N-terminal lobe of molluscan troponin C in troponin regulation.
Archives of biochemistry and biophysics - 1 Apr 2005
Doi Teppei, Satoh Ai, Tanaka Hiroyuki, Inoue Akira, Yumoto Fumiaki, Tanokura Masaru, Ohtsuki Iwao, Nishita Kiyoyoshi, Ojima Takao
Abstract excerpt
Ca(2+)-binding sites I and II in the N-terminal lobe of molluscan troponin C (TnC) have lost the ability to bind Ca(2+) due to substitutions of the amino acid residues responsible for Ca(2+) liganding. To evaluate the functional importance of the Ca(2+)-deficient N-terminal lobe in the Ca(2+)-regulatory function of molluscan troponin, we constructed chimeric TnCs comprising the N-terminal lobes from rabbit fast...
Topics
- Animals
- Base Sequence
- Binding Sites
- Ca(2+) Mg(2+)-ATPase
- Calcium
- Cations, Divalent
- Chimera
- Molecular Sequence Data
- Mollusca
- Mutation
- Peptides
- Rabbits
- Sequence Homology, Amino Acid
- Spectrophotometry, Ultraviolet