Article
Differential recovery of Ca2+ binding activity in mutated EF-hands of cardiac troponin C.
The Journal of biological chemistry - 15 Nov 1993
Dotson D G, Putkey J A
Abstract excerpt
Previous studies showed that conversion of the first Ca2+ ligand in Ca(2+)-binding sites III and IV from Asp to Ala decreased the affinity of cardiac TnC (cTnC) for the thin filament. Here, the functional consequences of mutation of the second ligand in the Ca(2+)-binding sites of cTnC were determined. Equilibrium dialysis and Tyr fluorescence studies showed that conversion of the second Ca2+ ligand to Ala...
Topics
- Adenosine Triphosphatases
- Amino Acid Sequence
- Animals
- Binding Sites
- Calcium
- Fluorescence
- Molecular Sequence Data
- Mutation
- Myocardium
- Rabbits
- Troponin
- Troponin C