Article
Sequencing analysis of RNA and DNA of exons 1 through 11 shows p53 gene alterations to be present in almost 100% of head and neck squamous cell cancers.
Laboratory investigation; a journal of technical methods and pathology - 1 Mar 1999
Kropveld A, Rozemuller E H, Leppers F G, Scheidel K C, de Weger R A, Koole R, Hordijk G J, Slootweg P J, Tilanus M G
Abstract excerpt
Data on p53 alterations in human cancers are mainly based on studies restricted to the core domain (exons 5-9), because mutations outside this region are assumed to be rare. To test this assumption, we studied 25 consecutive patients with primary, untreated head and neck squamous cell carcinoma (HNSCC) with a p53 mutation analysis strategy that consists of sequencing all 11 p53 exons and the complete p53 mRNA....
Topics
- Amino Acid Sequence
- Base Sequence
- Cadaver
- Carcinoma, Squamous Cell
- DNA, Neoplasm
- Exons
- Female
- Head and Neck Neoplasms
- Humans
- Immunohistochemistry
- Male
- Mutation
- Point Mutation