Article
Combining one-step Sanger sequencing with phasing probe hybridization for HLA class I typing yields rapid, G-group resolution predicting 99% of unique full length protein sequences.
HLA - 1 Feb 2017
Tu Bin, Masaberg Carly, Hou Lihua, Behm Daniel, Brescia Peter, Cha Nuri, Kariyawasam Kanthi, Lee Jar How, Nong Thoa, Sells John, Tausch Paul, Yang Ruyan, Ng Jennifer, Hurley Carolyn Katovich
Abstract excerpt
BACKGROUND: Sanger-based DNA sequencing of exons 2+3 of HLA class I alleles from a heterozygote frequently results in two or more alternative genotypes. This study was undertaken to reduce the time and effort required to produce a single high resolution HLA genotype. MATERIALS AND METHODS: Samples were typed in parallel by Sanger sequencing and oligonucleotide probe hybridization. This workflow, together with...
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