Article
Advancing allele group-specific amplification of the complete HLA-C gene--isolation of novel alleles from three allele groups (C*04, C*07 and C*08).
Tissue antigens - 1 Oct 2013
Cisneros E, Martínez-Pomar N, Vilches M, Martín P, de Pablo R, Nuñez Del Prado N, Nieto A, Matamoros N, Moraru M, Vilches C
Abstract excerpt
A variety of strategies have been designed for sequence-based HLA typing (SBT) and for the isolation of new human leucocyte antigen (HLA) alleles, but unambiguous characterization of complete genomic sequences remains a challenge. We recently reported a simple method for the group-specific amplification (GSA) and sequencing of a full-length C*04 genomic sequence in isolation from the accompanying allele. Here we...
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