Metal-site disruption or global destabilization?
For mutation effects on metal ion binding, loss of binding can reflect direct disruption of coordinating residues or broader destabilization of the protein. Does mCSM-metal provide outputs or benchmarks that separate these explanations, for example by comparing predicted binding effects with independent folding or stability measurements? Without that control, an apparent site-specific effect may remain compatible with loss of the folded binding-competent state.
