Genome editing in mammals using CRISPR type I-D nuclease

Abstract excerpt

<h4>SUMMARY</h4> Adoption of the CRISPR-Cas system has revolutionized genome engineering in recent years; however, application of genome editing with CRISPR type I—the most abundant CRISPR system in bacteria—has been less developed. Type I systems in which Cas3 nuclease degrades the target DNA are known; in contrast, for the sub-type CRISPR type I-D (TiD), which lacks a typical Cas3 nuclease in its cascade, the m...

Topics

Open a Topic to create a Post that cites this publication.

• CRISPR and Genetic Engineering
• CRISPR-Associated Proteins
• CRISPR-Cas Systems
• Endodeoxyribonucleases
• Gene Editing
• HEK293 Cells
• Mutagenesis
• Mutation

Identifiers and source

Literature Corpus work

fdc38e26-7bb2-5a29-80bb-0f6665f4e895

DOI

10.1101/2020.03.14.991976