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Application of CRISPR/Cas9 for biomedical discoveries

20 Jun 2015

Abstract excerpt

The Clustered Regions of Interspersed Palindromic Repeats-Cas9 (CRISPR/Cas9), a viral defense system found in bacteria and archaea, has emerged as a tour de force genome editing tool. The CRISPR/Cas9 system is much easier to customize and optimize because the site selection for DNA cleavage is guided by a short sequence of RNA rather than an engineered protein as in the systems of zinc finger nucleases (ZFN),...

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PMID: 26137216
DOI: 10.1186/s13578-015-0027-9

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