Fluorescent protein-based ticker tapes for multiplexed recordings of transcriptional histories in single cells in culture and in vivo

Abstract excerpt

Recording and imaging of promoter activities in real time is critical for deciphering cellular states and dynamic signaling crosstalk, but technologies capable of simultaneously capturing multiple transient events in living cells are lacking. Here, we custom-design fluorescent protein-based ticker tapes (FPTT) for multiplexed and longitudinal recording of physiological activities in single cells. FPTT integrates m...

Topics

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• Aptamers, Nucleotide
• Cell Cycle
• Chromatin
• CRISPR-Cas Systems
• Fluorescent Antibody Technique
• Gene Regulatory Networks
• Molecular Imaging
• Single-Cell Analysis

Identifiers and source

Literature Corpus work

faa2f56b-ad6b-5dfa-a7bb-d6b4a3a73606

DOI

10.1101/2025.09.08.675004