Article

Near 100% efficient homology-dependent genome engineering in the human fungal pathogen <i>Cryptococcus neoformans</i>

2025-02-18

Abstract excerpt

<h4>ABSTRACT</h4> We recently described CRISPR/Cas9-based short homology-dependent genome engineering in the human fungal pathogen Cryptococcus neoformans , a haploid budding yeast that is the most common cause of fungal meningitis and an emerging model organism. This was achieved by electroporation of strains stably expressing a codon-optimized Cas9 with two separate DNA molecules, one encoding a selectable mar...

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Literature Corpus work: f9264a7a-3570-5439-8d44-f3da2b87396e
DOI: 10.1101/2025.02.14.638277

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