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Analysis of a Rpb2 mutant in <i>Schizosaccharomyces pombe</i> reveals a non-canonical role for Elp1 in regulating RNAi-dependent heterochromatin assembly

2025-07-05

Abstract excerpt

Heterochromatin is a repressive epigenetic state that suppresses transcription and safeguards genomic integrity. However, the full mechanism of how it is regulated remains elusive. Here, we focus on a previously described Pol II variant called rpb2-N44Y, which has a single substitution mutation within the Rpb2 subunit of Pol II that reduces RNAi-dependent heterochromatin. Through CRISPR-mediated site-directed mut...

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Literature Corpus work
f8cd7f24-cf8d-571f-8cc7-56f4420eaa22
DOI
10.1101/2025.07.02.662331
Open publication

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Analysis of a Rpb2 mutant in <i>Schizosaccharomyces pombe</i> reveals a non-canonical role for Elp1 in regulating RNAi-dependent heterochromatin assemblyDOI 10.1101/2025.07.02.662331
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