Article

Image-based pooled whole genome CRISPR screening for Parkin and TFEB subcellular localization

2020-07-02

Abstract excerpt

Genome-wide CRISPR screens have transformed our ability to systematically interrogate human gene function, but are currently limited to a subset of cellular phenotypes. We report a novel pooled screening approach for a wider range of cellular and subtle subcellular phenotypes. Machine learning and convolutional neural network models are trained on the subcellular phenotype to be queried. Genome-wide screening then...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: f8c0c25d-e88a-5b30-a12e-62b9ad07c776
DOI: 10.1101/2020.07.02.184390

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Image-based pooled whole genome CRISPR screening for Parkin and TFEB subcellular localizationDOI 10.1101/2020.07.02.184390Read

Image-based pooled whole-genome CRISPRi screening for subcellular phenotypes.Semantic proximity Read

Pooled image-base screening of mitochondria with microraft isolation distinguishes pathogenic mitofusin 2 mutations.Semantic proximity Read

Large-scale image-based profiling of single-cell phenotypes in arrayed CRISPR-Cas9 gene perturbation screens.Semantic proximity Read

A CRISPR-CAS9 high throughput machine-learning platform for modulation of genes involved in Parkinson’s disease-associated PINK1-mitophagy in iPSC-derived dopaminergic neuronsSemantic proximity Read

Mitochondrial Phenotypes Distinguish Pathogenic MFN2 Mutations by Pooled Functional Genomics ScreenSemantic proximity Read

High-content imaging-based pooled CRISPR screens in mammalian cells.Semantic proximity Read

Cell organization and disturbance-mapping using image activated cell-profiling (CODIAC)Semantic proximity Read

Machine learning–based image analysis of Parkinson’s disease iPS cell-derived neurons predicts genotype and reveals mitochondria–lysosome abnormalitiesSemantic proximity Read

Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosisSemantic proximity Read

Genetically Encoded and Modular SubCellular Organelle Probes (GEM-SCOPe) reveal lysosomal and mitochondrial dysfunction driven by <i>PRKN</i> knockoutSemantic proximity Read

A Pooled Cell Painting CRISPR Screening Platform Enables de novo Inference of Gene Function by Self-supervised Deep LearningSemantic proximity Read

Organellomics: AI-driven deep organellar phenotyping reveals novel ALS mechanisms in human neuronsSemantic proximity Read