Article

A CRISPR-CAS9 high throughput machine-learning platform for modulation of genes involved in Parkinson’s disease-associated PINK1-mitophagy in iPSC-derived dopaminergic neurons

2025-06-15

Abstract excerpt

Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterised by the loss of dopaminergic neurons, driven by complex molecular mechanisms that are not fully understood. To address this issue, we have developed a novel high-content phenotypic screening platform using human induced pluripotent stem cell-derived dopaminergic neurons to investigate the PINK1-PARKIN mitophagy pathway, a critical pr...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 12edcb27-59d2-524f-90e3-c6e0c1aa9c54
DOI: 10.1101/2025.06.10.658840

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A CRISPR-CAS9 high throughput machine-learning platform for modulation of genes involved in Parkinson’s disease-associated PINK1-mitophagy in iPSC-derived dopaminergic neuronsDOI 10.1101/2025.06.10.658840Read

Systematic Functional Analysis of PINK1 and PRKN Coding VariantsSemantic proximity Read

CRISPR System: A High-throughput Toolbox for Research and Treatment of Parkinson's Disease.Semantic proximity Read

High-content phenotypic screen to identify small molecule enhancers of Parkin-dependent ubiquitination and mitophagy.Semantic proximity Read

High-content phenotypic screen to identify small molecule enhancers of Parkin-dependent ubiquitination and mitophagySemantic proximity Read

An isogenic single-cell atlas of familial Parkinson’s disease mutations reveals convergent changes in dopamine neuronsSemantic proximity Read

Single-cell transcriptomics of human iPSC differentiation dynamics reveal a core molecular network of Parkinson's disease.Semantic proximity Read

Targeting mitophagy in Parkinson's disease.Semantic proximity Read

Generation of PRKN and PINK1-KO and double KO cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editingSemantic proximity Read

iPS models of Parkin and PINK1.Semantic proximity Read

In vitro genome editing rescues parkinsonism phenotypes in induced pluripotent stem cells-derived dopaminergic neurons carrying LRRK2 p.G2019S mutation.Semantic proximity Read

PRKN-linked familial Parkinson’s disease: cellular and molecular mechanisms of disease-linked variantsSemantic proximity Read

iSCORE-PD: an isogenic stem cell collection to research Parkinson’s DiseaseSemantic proximity Read