Article

Interaction of human CRX and NRL in live HEK293T cells measured using fluorescence resonance energy transfer (FRET)

2021-10-19

Abstract excerpt

CRX and NRL are retina-specific transcription factors that control rod photoreceptor differentiation and synergistically activate rod phototransduction gene expression. Previous experiments showed they interact in vitro and in yeast two-hybrid assays. Here, we examined CRX-NRL interaction in live HEK293T cells using two fluorescence resonance energy transfer (FRET) approaches: confocal microscopy and flow cytomet...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: f6ee4421-5502-545f-91a7-191f8d42174d
DOI: 10.1101/2021.10.19.465002

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Interaction of human CRX and NRL in live HEK293T cells measured using fluorescence resonance energy transfer (FRET)DOI 10.1101/2021.10.19.465002Read

Mutations in the DNA-binding domain of NR2E3 affect in vivo dimerization and interaction with CRX.Semantic proximity Read

Molecular basis of CRX/DNA recognition and stoichiometry at the Ret4 response element.Semantic proximity Read

Functional Domains of the Cone-Rod Homeobox (CRX) Transcription FactorSemantic proximity Read

Neurogenin1 effectively reprograms cultured chick retinal pigment epithelial cells to differentiate toward photoreceptors.Semantic proximity Read

Mechanistically distinct mouse models for CRX-associated retinopathy.Semantic proximity Read

Functional analysis of the rod photoreceptor cGMP phosphodiesterase alpha-subunit gene promoter: Nrl and Crx are required for full transcriptional activity.Semantic proximity Read

Structural and functional analysis of the human Cone-rod homeobox transcription factorSemantic proximity Read

Differential dimerization of variants linked to enhanced S-cone sensitivity syndrome (ESCS) located in the NR2E3 ligand-binding domain.Semantic proximity Read

Developmental genome-wide occupancy analysis of bZIP transcription factor NRL uncovers the role of c-Jun in early differentiation of rod photoreceptors in the mammalian retinaSemantic proximity Read

Iris-derived cells from adult rodents and primates adopt photoreceptor-specific phenotypes.Semantic proximity Read

Heterodimers of photoreceptor-specific nuclear receptor (PNR/NR2E3) and peroxisome proliferator-activated receptor-γ (PPARγ) are disrupted by retinal disease-associated mutations.Semantic proximity Read

Functional dissection of the promoter of the interphotoreceptor retinoid-binding protein gene: the cone-rod-homeobox element is essential for photoreceptor-specific expression in vivo.Semantic proximity Read