Article

A simple, cost-effective and extraction-free molecular diagnostic test for sickle cell disease in noninvasive buccal swab specimen for a limited-resource setting

2022-06-18

Abstract excerpt

Sickle cell disease (SCD) is the most prevalent life threatening blood monogenic disorder. Currently, there is no cure available, apart from bone marrow transplantation. Early and efficient diagnosis of SCD is key to disease management which would make considerable strides in alleviating morbidity and reducing mortality. However, the cost and complexity of diagnostic procedures like Sanger sequencing impede the ea...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: f5e81e12-efea-5c1a-8045-1d4edceec710
DOI: 10.1101/2022.06.14.22276141

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A simple, cost-effective and extraction-free molecular diagnostic test for sickle cell disease in noninvasive buccal swab specimen for a limited-resource settingDOI 10.1101/2022.06.14.22276141Read

Scalable noninvasive amplicon-based precision sequencing (SNAPseq) for genetic diagnosis and screening of β-thalassemia and sickle cell disease using a next-generation sequencing platformSemantic proximity Read

Allele-Specific Recombinase Polymerase Amplification to Detect Sickle Cell Disease in Low-Resource Settings.Semantic proximity Read

One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics.Semantic proximity Read

Noninvasive and Accurate Detection of Hereditary Hearing Loss Mutations with Buccal Swab Based on Droplet Digital PCR.Semantic proximity Read

Miniaturized sealed-tube allele-specific PCR.Semantic proximity Read

Isothermal single nucleotide polymorphism genotyping and direct PCR from whole blood using a novel whole-blood lysis buffer.Semantic proximity Read

A portable molecular laboratory for rapid genotyping in the field: application to sickle cell diseaseSemantic proximity Read

Utility of First-Line Diagnostic Tests and Molecular Methods for Accurate Characterization of Sickle Cell Disorders.Semantic proximity Read

Optimized Droplet Digital PCR Assay on Cell-Free DNA Samples for Non-Invasive Prenatal Diagnosis: Application to Beta-Thalassemia.Semantic proximity Read

A multiplexed, allele-specific recombinase polymerase amplification assay with lateral flow readout for sickle cell disease detection.Semantic proximity Read

SNP genotyping using single-tube fluorescent bidirectional PCR.Semantic proximity Read

Single Lysis-Salting Out Method of Genomic DNA Extraction From Dried Blood Spots.Semantic proximity Read