Article
Isothermal single nucleotide polymorphism genotyping and direct PCR from whole blood using a novel whole-blood lysis buffer.
Molecular diagnosis & therapy - 1 Dec 2009
Victor Sylvia T, Lezhava Alexander, Ishidao Takefumi, Endo Ryuta, Mitani Yasumasa, Kawaoka Yoshihiro, Hayashizaki Yoshihide
Abstract excerpt
Cell lysis and subsequent release of genomic DNA is an ongoing dilemma for molecular biological techniques. In most cases, technologies such as PCR and other amplification techniques require DNA extraction and purification steps. The Smart Amplification Process Version 2 (SmartAmp2) is an isothermal and integrated amplification technology that eliminates the need for time-consuming sample preparation for the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.