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Article

Single Lysis-Salting Out Method of Genomic DNA Extraction From Dried Blood Spots.

Journal of clinical laboratory analysis - 1 Nov 2016

Shaik Muntaj, Shivanna Devaraju Kuramkote, Kamate Mahesh, Ab Vedamurthy, Tp Kruthika-Vinod

Abstract excerpt

BACKGROUND: Dried blood spots (DBS) are an important form of bio-sampling and valuable approach for storing blood samples for genetic studies. This has necessitated in developing an effective protocol to isolate genomic DNA (gDNA) from DBS samples.In this study, we have elucidated a dependable and non-hazardous "single lysis-salting out" (SLSO) protocol of gDNA extraction from DBS and compared against the...

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Identifiers

PMID: 27074880
DOI: 10.1002/jcla.21972
PMCID: PMC6806700

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