Article

Mapping variation in the morphological landscape of human cells with optical pooled CRISPRi screening

2022-12-27

Abstract excerpt

<h4>ABSTRACT</h4> The contributions of individual genes to cell-scale morphology and cytoskeletal organization are challenging to define due to the wide intercellular variation of these complex phenotypes. We leveraged the controlled nature of image-based pooled screening to assess the impact of CRISPRi knockdown of 366 genes on cell and nuclear morphology in human U2OS osteosarcoma cells. Screen scale-up was fac...

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Literature Corpus work: f3858c14-0535-55ce-bf76-4a6a6df67252
DOI: 10.1101/2022.12.27.522042

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