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Article

Disruption of exogenous eGFP gene using RNA-guided endonuclease in bovine transgenic somatic cells.

Zygote (Cambridge, England) - 1 Dec 2015

Choi WooJae, Yum SooYoung, Lee SongJeon, Lee WonWu, Lee JiHyun, Kim SeokJoong, Koo OkJae, Lee ByeongChun, Jang Goo

Abstract excerpt

Genome-editing technologies are considered to be an important tool for generating gene knockout cattle models. Here, we report highly efficient disruption of a chromosomally integrated eGFP gene in bovine somatic cells using RNA-guided endonucleases, a new class of programmable nucleases developed from a bacterial Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system. In the present...

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PMID: 25424059
DOI: 10.1017/S096719941400063X

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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