Article
Disruption of exogenous eGFP gene using RNA-guided endonuclease in bovine transgenic somatic cells.
Zygote (Cambridge, England) - 1 Dec 2015
Choi WooJae, Yum SooYoung, Lee SongJeon, Lee WonWu, Lee JiHyun, Kim SeokJoong, Koo OkJae, Lee ByeongChun, Jang Goo
Abstract excerpt
Genome-editing technologies are considered to be an important tool for generating gene knockout cattle models. Here, we report highly efficient disruption of a chromosomally integrated eGFP gene in bovine somatic cells using RNA-guided endonucleases, a new class of programmable nucleases developed from a bacterial Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system. In the present...
Topics
- Animals
- Animals, Genetically Modified
- Blastocyst
- CRISPR-Cas Systems
- Cattle
- Cells, Cultured
- Endonucleases
- Female
- Fibroblasts
- Gene Knockout Techniques
- Green Fluorescent Proteins