Article

Efficient Generation of Humanized Cloned Cattle via Embryonic Stem Cell-Mediated Dual-Round Site-Specific Editing

2025-12-23

Abstract excerpt

Transgenic cloning allows genetic modification in cattle, yet its translational potential is constrained by low cloning efficiency and limited gene-editing capacity, due largely to the restricted proliferative ability of somatic donor cells. Pluripotent stem cells (PSCs), with their unlimited self-renewal ability, represent a promising alternative to overcome these bottlenecks. Here, we first established stable bo...

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Literature Corpus work: 8c7ddd80-85bb-5d37-878b-b09e5b1c66b5
DOI: 10.64898/2025.12.20.695363

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