Article
Evaluation of mutation rates, mosaicism and off target mutations when injecting Cas9 mRNA or protein for genome editing of bovine embryos.
Scientific reports - 18 Dec 2020
Hennig Sadie L, Owen Joseph R, Lin Jason C, Young Amy E, Ross Pablo J, Van Eenennaam Alison L, Murray James D
Abstract excerpt
The CRISPR/Cas9 genome editing tool has the potential to improve the livestock breeding industry by allowing for the introduction of desirable traits. Although an efficient and targeted tool, the CRISPR/Cas9 system can have some drawbacks, including off-target mutations and mosaicism, particularly when used in developing embryos. Here, we introduced genome editing reagents into single-cell bovine embryos to...
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