Evaluation of mutation rates, mosaicism and off target mutations when injecting Cas9 mRNA or protein for genome editing of bovine embryos.

Abstract excerpt

The CRISPR/Cas9 genome editing tool has the potential to improve the livestock breeding industry by allowing for the introduction of desirable traits. Although an efficient and targeted tool, the CRISPR/Cas9 system can have some drawbacks, including off-target mutations and mosaicism, particularly when used in developing embryos. Here, we introduced genome editing reagents into single-cell bovine embryos to...

Topics

• Animals
• CRISPR-Associated Protein 9
• CRISPR-Cas Systems
• Cattle
• Embryo, Mammalian
• Gene Editing
• Genome
• Mosaicism
• Mutation
• Mutation Rate
• RNA, Messenger