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Article

Efficient gene targeting in mouse zygotes mediated by CRISPR/Cas9-protein.

Transgenic research - 1 Apr 2017

Jung Chris J, Zhang Junli, Trenchard Elizabeth, Lloyd Kent C, West David B, Rosen Barry, de Jong Pieter J

Abstract excerpt

The CRISPR/Cas9 system has rapidly advanced targeted genome editing technologies. However, its efficiency in targeting with constructs in mouse zygotes via homology directed repair (HDR) remains low. Here, we systematically explored optimal parameters for targeting constructs in mouse zygotes via HDR using mouse embryonic stem cells as a model system. We characterized several parameters, including single guide...

Topics

Alleles
Animals
CRISPR-Cas Systems
DNA End-Joining Repair
Exons
Gene Targeting
Homologous Recombination
Mice
RNA, Guide, CRISPR-Cas Systems
RNA, Messenger
Zygote

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Identifiers

PMID: 27905063
DOI: 10.1007/s11248-016-9998-5
PMCID: PMC5350237

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-27905063-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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