Article

Rational design of Cas9 ribonucleoprotein with a “gRNA-shRNA” for multidimensional genome manipulation and enhanced homology-directed repair

2022-03-17

Abstract excerpt

Gene perturbation approaches have evolved as powerful tools for understanding the function of genes and curing inherited diseases. Here, we develop a method that combines the merits of RNAi and CRISPR technology by rational design of Cas9 ribonucleoprotein (RNP) with a “gRNA-shRNA” component. The RNP, termed Cas9-RNAi, has a gRNA containing a 3’ extension that can be processed to a functional siRNA via dorsha/dice...

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Literature Corpus work: 97157dca-09cc-5452-9b90-056ec25f5904
DOI: 10.1101/2022.03.17.484717

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