Article

Optimized cross-linking mass spectrometry for in situ interaction proteomics

2018-08-17

Abstract excerpt

Recent development of mass spectrometer cleavable protein cross-linkers and algorithms for their spectral identification now permits large-scale cross-linking mass spectrometry (XL-MS). Here, we optimized the use of cleavable disuccinimidyl sulfoxide (DSSO) cross-linker for labeling native protein complexes in live human cells. We applied a generalized linear mixture model to calibrate cross-link peptide-spectra m...

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Literature Corpus work: e91ba5e8-4150-54eb-b095-480ac90d0fe7
DOI: 10.1101/393892

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