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Article

Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents.

Molecular & cellular proteomics : MCP - 1 Dec 2004

Ross Philip L, Huang Yulin N, Marchese Jason N, Williamson Brian, Parker Kenneth, Hattan Stephen, Khainovski Nikita, Pillai Sasi, Dey Subhakar, Daniels Scott, Purkayastha Subhasish, Juhasz Peter, Martin Stephen, Bartlet-Jones Michael, He Feng, Jacobson Allan, Pappin Darryl J

Abstract excerpt

We describe here a multiplexed protein quantitation strategy that provides relative and absolute measurements of proteins in complex mixtures. At the core of this methodology is a multiplexed set of isobaric reagents that yield amine-derivatized peptides. The derivatized peptides are indistinguishable in MS, but exhibit intense low-mass MS/MS signature ions that support quantitation. In this study, we have...

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PMID: 15385600
DOI: 10.1074/mcp.M400129-MCP200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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