Article

Click-linking: a cell-compatible protein crosslinking method based on click chemistry

2024-12-23

Abstract excerpt

Crosslinking mass spectrometry (XL-MS) has the potential to map the human interactome at high resolution and with high fidelity, replacing indirect, error prone sampling methods such as affinity pulldown MS. However, the sampling depth of XL-MS remains stubbornly low. We present a novel crosslinking strategy that splits the crosslinking reaction into two sequential and orthogonal coupling events. The method involv...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 7c2df3d7-422a-5f5a-941d-6afa8273c267
DOI: 10.1101/2024.12.23.630117

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Click-linking: a cell-compatible protein crosslinking method based on click chemistryDOI 10.1101/2024.12.23.630117Read

Aggregate Interactome Based on Protein Cross-linking Interfaces Predicts Drug Targets to Limit Aggregation in Neurodegenerative DiseasesSemantic proximity Read

Mapping the Human Proteome with Physical Access to DNASemantic proximity Read

Single-Cell Protein Interactomes by the Proximity Network AssaySemantic proximity Read

Development and Comparative Evaluation of Endolysosomal Proximity Labeling-based Proteomic Methods in Human iPSC-derived NeuronsSemantic proximity Read

Characterization of PROTAC specificity and endogenous protein interactomes using ProtacIDSemantic proximity Read

High affinity cross-context cellular assays reveal novel protein-protein interactions of peripheral myelin protein of 22 kDaSemantic proximity Read

Multiplex mapping of protein-protein interaction interfacesSemantic proximity Read

Simultaneous profiling of native-state proteomes and transcriptomes of neural cell types using proximity labelingSemantic proximity Read

Spatiotemporally resolved mapping of extracellular proteomes via in vivo-compatible TyroIDSemantic proximity Read

SLAPSHOT reveals rapid dynamics of extracellularly exposed proteome in response to calcium-activated plasma membrane phospholipid scramblingSemantic proximity Read

An atlas of protein-protein interactions across mammalian tissuesSemantic proximity Read

Benchmarking of proximity-dependent biotinylation enzymes across cellular compartments and time windowsSemantic proximity Read