Article

Generation of Cerebral Organoids with Enriched Cortical Cellular Diversity and Outer Radial Glial Cell Identity from Human Pluripotent Stem Cells

2022-06-13

Abstract excerpt

<title>Abstract</title> <p>Cerebral organoids display broad regional heterogeneity and limited capacity to generate cortical cell diversity due to incompetent patterning signals within early developing organoid neural stem cells (NSCs). Here we describe a method to overcome this limitation by applying a short and early dual SMAD/WNT inhibition course, which results in enrichment for cortical NSCs and their progen...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e75b6ed3-1989-5b23-8bf8-09806283e498
DOI: 10.21203/rs.3.pex-1877/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Generation of Cerebral Organoids with Enriched Cortical Cellular Diversity and Outer Radial Glial Cell Identity from Human Pluripotent Stem CellsDOI 10.21203/rs.3.pex-1877/v1Read

Single-cell multiomics atlas of organoid development uncovers longitudinal molecular programs of cellular diversification of the human cerebral cortexSemantic proximity Read

Brain regional identity and cell type specificity landscape of human cortical organoid modelsSemantic proximity Read

Generation of human cerebral organoids with a structured outer subventricular zoneSemantic proximity Read

Human cortical organoids expose a differential function of GSK3 on direct and indirect neurogenesisSemantic proximity Read

Robust Production of Uniform Human Cerebral Organoids from Pluripotent Stem CellsSemantic proximity Read

Lineage recording reveals dynamics of cerebral organoid regionalizationSemantic proximity Read

Human basal radial glia morphotypes are transcriptionally distinct and exhibit different cell fate determinationSemantic proximity Read

Unified Generation of Regionalized Neural Organoids from Single-Lumen NeuroepitheliumSemantic proximity Read

Self-organizing Single-Rosette Brain Organoids from Human Pluripotent Stem CellsSemantic proximity Read

Brain organoid-on-a-chip to create multiple domains in forebrain organoidsSemantic proximity Read

Differentiation of cortical brain organoids and optic nerve-like structures from retinal confluent cultures of pluripotent stem cellsSemantic proximity Read

Validation of non-destructive morphology-based selection of cerebral cortical organoids by paired morphological and single-cell RNA-seq analysesSemantic proximity Read