Article

Product-stabilized filamentation by human glutamine synthetase allosterically tunes metabolic activity

2025-07-06

Abstract excerpt

To maintain metabolic homeostasis, enzymes must adapt to fluctuating nutrient levels through mechanisms beyond gene expression. Here, we demonstrate that human glutamine synthetase (GS) can reversibly polymerize into filaments aided by a composite binding site formed at the filament interface by the product, glutamine. Time-resolved cryo-electron microscopy (cryo-EM) confirms that glutamine binding stabilizes thes...

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Literature Corpus work: e6d46a40-2a90-5cf0-8405-5999c152e6b5
DOI: 10.1101/2025.07.04.663231

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