Article

Single-cell Individual Complete mtDNA Sequencing Uncovers Hidden Mitochondrial Heterogeneity in Human and Mouse Oocytes

2020-12-28

Abstract excerpt

The ontogeny and dynamics of mtDNA heteroplasmy remain unclear due to limitations of current mtDNA sequencing methods. We developed individual Mi tochondrial G enome seq uencing (iMiGseq) of full-length mtDNA for ultra-sensitive variant detection, complete haplotyping, and unbiased evaluation of heteroplasmy levels, all at the individual mtDNA molecule level. iMiGseq uncovers unappreciated levels of heteroplasm...

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Literature Corpus work: ddb275ec-62c0-554e-9f5f-6dd8940a403e
DOI: 10.1101/2020.12.28.424537

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