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Single-molecule mitochondrial DNA imaging reveals heteroplasmy dynamics shaped by developmental bottlenecks and selection in different organs <i>in vivo</i>

2025-01-25

Abstract excerpt

Mitochondrial DNA (mtDNA) occurs in many copies per cell, with cell-to-cell variability in mutation load, known as heteroplasmy. Developmental and age-related expansion of pathogenic mtDNA mutations contributes to mitochondrial and neurodegenerative disease pathogenesis. Here, we describe an approach for in situ sequence-specific detection of single mtDNA molecules (mtDNA-smFISH). We apply this method to visualiz...

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Literature Corpus work
7e7e3aa1-7e15-51d4-b137-9eebe4035af6
DOI
10.1101/2025.01.24.634671
Open publication

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Single-molecule mitochondrial DNA imaging reveals heteroplasmy dynamics shaped by developmental bottlenecks and selection in different organs <i>in vivo</i>DOI 10.1101/2025.01.24.634671
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