Article
Single-cell individual full-length mtDNA sequencing by iMiGseq uncovers unexpected heteroplasmy shifts in mtDNA editing.
Nucleic acids research - 8 May 2023
Bi Chongwei, Wang Lin, Fan Yong, Yuan Baolei, Ramos-Mandujano Gerardo, Zhang Yingzi, Alsolami Samhan, Zhou Xuan, Wang Jincheng, Shao Yanjiao, Reddy Pradeep, Zhang Pu-Yao, Huang Yanyi, Yu Yang, Izpisua Belmonte Juan Carlos, Li Mo
Abstract excerpt
The ontogeny and dynamics of mtDNA heteroplasmy remain unclear due to limitations of current mtDNA sequencing methods. We developed individual Mitochondrial Genome sequencing (iMiGseq) of full-length mtDNA for ultra-sensitive variant detection, complete haplotyping, and unbiased evaluation of heteroplasmy levels, all at the individual mtDNA molecule level. iMiGseq uncovered unappreciated levels of heteroplasmic...
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