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C-terminal HA tags compromise function and exacerbate phenotypes of <i>Saccharomyces cerevisiae</i> Bloom’s helicase homolog Sgs1 SUMOylation-associated mutants

2020-04-24

Abstract excerpt

The Sgs1 helicase and Top3-Rmi1 decatenase form a complex that affects homologous recombination outcomes during the mitotic cell cycle and during meiosis. Previous studies have reported that Sgs1-Top3-Rmi1 function is regulated by SUMOylation that is catalyzed by the Smc5-Smc6-Mms21 complex. These studies used strains in which SGS1 was C-terminally tagged with three or six copies of a human influenza hemagglutini...

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Literature Corpus work
dc7eaabd-f7d2-5b8f-b82b-087d38dab7ab
DOI
10.1101/2020.04.23.058578
Open publication

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C-terminal HA tags compromise function and exacerbate phenotypes of <i>Saccharomyces cerevisiae</i> Bloom’s helicase homolog Sgs1 SUMOylation-associated mutantsDOI 10.1101/2020.04.23.058578
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