Article

Single-Stranded DNA with Internal Base Modifications Mediates Highly Efficient Gene Insertion in Primary Cells

2024-02-01

Abstract excerpt

<h4>ABSTRACT</h4> Single-stranded DNA (ssDNA) templates along with Cas9 have been used for gene insertion but suffer from low efficiency. Here, we show that ssDNA with chemical modifications in 10-17% of internal bases (eDNA) is compatible with the homologous recombination machinery. Moreover, eDNA templates improve gene insertion by 2-3 fold compared to unmodified and end-modified ssDNA in airway basal stem cell...

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Literature Corpus work: d94ae758-f43b-5fa2-9fef-c51108e9122e
DOI: 10.1101/2024.02.01.578476

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