Article

Long-read transcriptomics of purified human cortical cell types exposes glial isoform complexity and disease-relevant transcript architecture

2025-11-25

Abstract excerpt

Alternative splicing generates extraordinary transcriptomic complexity in the human brain, yet the full-length isoform landscape across human cortical cell types remains uncharted. Combining fluorescence-activated nuclei sorting with long- and short-read RNA sequencing, we generated isoform-resolved transcriptomes for five major lineages of the adult human prefrontal and orbitofrontal cortex: GABAergic neurons, gl...

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Literature Corpus work: d55712f6-0f48-5518-980e-56c77d2002bb
DOI: 10.1101/2025.11.25.690524

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