Article

Using deep long-read RNAseq in Alzheimer’s disease brain to assess medical relevance of RNA isoform diversity

2023-08-07

Abstract excerpt

Due to alternative splicing, human protein-coding genes average over eight RNA isoforms, resulting in nearly four distinct protein coding sequences per gene. Long-read RNAseq (IsoSeq) enables more accurate quantification of isoforms, shedding light on their specific roles. To assess the medical relevance of measuring RNA isoform expression, we sequenced 12 aged human frontal cortices (6 Alzheimer’s disease cases a...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 86abac3d-c05b-5e90-bf7f-4dcc1b3e912b
DOI: 10.1101/2023.08.06.552162

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Using deep long-read RNAseq in Alzheimer’s disease brain to assess medical relevance of RNA isoform diversityDOI 10.1101/2023.08.06.552162Read

Surveying the landscape of RNA isoform diversity and expression across 9 GTEx tissues using long-read sequencing dataSemantic proximity Read

RNA isoform diversity, splicing variants, and switching in single cells of the Alzheimer’s disease brainSemantic proximity Read

Long-read sequencing reveals the RNA isoform repertoire of neuropsychiatric risk genes in human brainSemantic proximity Read

Long-read proteogenomic atlas of human neuronal differentiation reveals isoform diversity informing neurodevelopmental risk mechanismsSemantic proximity Read

Long-read transcriptomics of purified human cortical cell types exposes glial isoform complexity and disease-relevant transcript architectureSemantic proximity Read

Accurate strand-specific long-read transcript isoform discovery and quantification at bulk, single-cell, and single-nucleus resolutionSemantic proximity Read

Cell-Type-Resolved Isoform Atlas of Human Tissues Reveals Age and Alzheimer’s Disease-Associated Splicing ChangesSemantic proximity Read

Long-Read RNA-sequencing reveals transcript-specific regulation in human-derived cortical neuronsSemantic proximity Read

SCOTCH: isoform-level characterization of gene expression through long-read single-cell RNA sequencingSemantic proximity Read

Full-length transcript sequencing of human and mouse identifies widespread isoform diversity and alternative splicing in the cerebral cortexSemantic proximity Read

Direct RNA Sequencing reveals epitranscriptomic regulation of brain cells and Alzheimer’s Disease pathologySemantic proximity Read

Single-cell atlas of transcriptomic vulnerability across multiple neurodegenerative and neuropsychiatric diseasesSemantic proximity Read