Article

Highly sensitive mapping of <i>in vitro</i> type II topoisomerase DNA cleavage sites with SHAN-seq

2024-05-17

Abstract excerpt

Type II topoisomerases (topos) are a ubiquitous and essential class of enzymes that form transient enzyme-bound double-stranded breaks on DNA called cleavage complexes. The location and frequency of these cleavage complexes on DNA is important for cellular function, genomic stability, and a number of clinically important anticancer and antibacterial drugs, e.g., quinolones. We developed a simple high-accuracy end-...

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Literature Corpus work: d51926e5-949b-597a-91d3-afb322c3a245
DOI: 10.1101/2024.05.17.594727

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