Article

Nucleoporin-binding nanobodies that either track or inhibit nuclear pore complex assembly

2023-09-14

Abstract excerpt

Nuclear pore complex (NPC) biogenesis is a still enigmatic example of protein self-assembly. We now introduce several cross-reacting anti-Nup nanobodies for imaging intact NPCs from frog to human. We further report a simplified assay that directly tracks postmitotic NPC assembly by added labeled anti-Nup nanobodies. In interphase, NPCs are inserted into a pre-existing nuclear envelope. This makes it difficult to m...

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Literature Corpus work: d4c585a1-bde9-5bbb-95d4-074ddb449e10
DOI: 10.1101/2023.09.12.557426

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