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Article

The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex.

Biochemical and biophysical research communications - 30 Sept 2016

Tie Hieng Chiong, Madugula Viswanadh, Lu Lei

Abstract excerpt

We report here an image-based method to quantify the stoichiometry of diffraction-limited sub-cellular protein complexes in vivo under spinning disk confocal microscopy. A GFP single molecule fluorescence standard was first established by immobilizing His-tagged GFP molecules onto the glass surface via nickel nitrilotriacetic acid functionalized polyethylene glycol. When endogenous nucleoporins were knocked down...

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PMID: 27613095
DOI: 10.1016/j.bbrc.2016.09.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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